Dr. Jason Maynes

Lab Description

Dr. Maynes's laboratory is located  in the new Peter Gilgan Centre for Research and Learning at the Hospital for Sick Children. He has 14 current lab members including research associates, technicians, graduate students and clinical fellows. The lab has over 40 collaborators including clinicians, surgeons, epidemiologists, engineers and basic scientists. The lab reserach focuses on three main areas that attempt to combine Dr. Maynes's clinical training in paediatrics and anaesthesia with his research training in biophysics: (1) the mechanism of anaesthetic action and anaesthetic off-targets, (2) proteins involved in mitochondrial dynamics and, (3) high-content imaging and image analysis. Methodologies span all spectrums, including X-ray crystallography, NMR, mammalian cell protein expression, fluorescence spectroscopy, high-resolution fluorescence imaging, high-content imaging, robotics, micro-injection, stem cell models, and animal models.

Research Focus

In a broad, overarching theme, the Maynes  lab focuses on investigating mechanisms of drug toxicity and damage. Much of the unpredictability of drug toxicity is due to inadequate pre-clinical models to identify cellular dysfunction that would require massive numbers to identify in Stage III/IV clinical trials, owing to the potential diversity of the clinical phenotype resulting from the underlying toxicity being observed on a whole-organism scale. This concept leads to another key issue with drug toxicity, specifically the development of assays to measure cellular dysfunction in more complex systems than at the single enzyme level, taking into consideration the cellular ecosystem using a systems biology approach. Mitochondria are the primary metabolic organelles in the cell and also play important roles in modulating cell health including when to induce cell death via several different pathways. This organelle then is often a key component of drug toxicity. The lab addresses these concepts by looking at new models and assays to predict toxicity (including the use of stem-cell derived tissues), and investigating fundamental concepts in mitochondrial form, function and health. Researchers in the Maynes Lab are working to link current concepts and problems in clinical medicine with research techniques to form an active translational medicine research program.

Selected Publications 

  1. Traister A, Li M, Aafaqi S, Lu M, Arab S, Radisic M, Gross G, Guido F, Sherret J, Verma S, Slorach C, Mertens L, Hui W, Roy A, Delgado-Olguín P, Hannigan G, Maynes JT, Coles JG. Integrin-linked kinase mediates force transduction in cardiomyocytes by modulating SERCA2a/PLN function. Nat Commun. 2014 Sep 11;5:4533. 
  2. Kato H, Fu YY, Zhu J, Wang L, Aafaqi S, Rahkonen O, Slorach C, Traister A, Leung CH, Chiasson D, Mertens L, Benson L, Weisel RD, Hinz B, Maynes JT, Coles JG, Caldarone CA. Pulmonary vein stenosis and the pathophysiology of "upstream" pulmonary veins. J Thorac Cardiovasc Surg. 2014 Jul;148(1):245-53. 
  3. Zhu J, Ide H, Fu YY, Teichert AM, Kato H, Weisel RD, Maynes JT, Coles JG, Caldarone CA. Losartan ameliorates "upstream" pulmonary vein vasculopathy in a piglet model of pulmonary vein stenosis. J Thorac Cardiovasc Surg. 2014 Dec;148(6):2550-7
  4. Vincent A, Forster N, Maynes JT, Paton TA, Billingsley G, Roslin NM, Ali A, Sutherland J, Wright T, Westall CA, Paterson AD, Marshall CR; FORGE Canada Consortium, Héon E. OTX2 mutations cause autosomal dominant pattern dystrophy of the retinal pigment epithelium. J Med Genet. 2014 Dec;51(12):797-805. 
  5. Sittampalam S, Eglen R, Ferguson S, Maynes JT, Olden K, Schrader L, Shelper T, Ferrer M. Three-Dimensional Cell Culture Assays: Are They More Predictive of In Vivo Efficacy than 2D Monolayer Cell-Based Assays? Assay Drug Dev Technol. 2015 Jun;13(5):254-61. 
Jason Maynes, PhD, MD

Jason Maynes, MD, PhD

E: jason.maynes@sickkids.ca
P: (416) 813-5924

 

http://biochemistry.utoronto.ca/person/jason-t-maynes/

 

Laboratory Members

Rick Bagshaw: Research Associate
Michael Tropak: Research Associate
Anouk Teichert: Research Associate
Ramesh Vanama: Lab Manager
Sandra Singhroy: Technician
Olga Chernysh: Technician
Julia Plakhotnik: Graduate Student
Manpreet Malhi: Graduate Student
Doorsa Tarazi: Graduate Student

 

 

 

 

 

 

 

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